RGF-PCR: A technique to isolate different copies of a multi-copy gene

摘要

Isolation of different copies of a multi-copy gene from the genome is a difficult process. To overcome the difficulty, a novel method known as 'Restricted Genomic Fraction-Polymerase Chain Reaction' (RGF-PCR) has been developed. RGF-PCR is a combination of fractionation of the restricted genome and PCR methods. The transposon Mariner-Like Elements (MLEs) occur as multi-copy genes in the silkworm, Bombyx mori genome. Using RGF-PCR method, isolation of different copies of the multi-copy MLE from different loci of the silkworm genome has been demonstrated. Polymorphic copies of MLEs have been isolated from restricted genomic fractions of silkworm, and the nature of polymorphism has been analysed and presented in this paper.