A rapid and efficient method for isolation of RNA from bark tissues of Hevea brasiliensis.

摘要

Four previously published protocols were examined for the isolation of RNA from Hevea brasiliensis bark tissues. As none of these yielded high quality RNA, a modified protocol was developed by combining certain steps from protocols developed by Logemann et al. and Shirzadegan et al. This simple and rapid procedure is designed to reduce the amount of insoluble polysaccharides and remove polyphenols by addition of polyvinyl polypyrrolidone [polyvidone]. An efficient yield of RNA suitable for poly(A)+enrichment and analysis of less-abundant transcripts in Hevea bark was maintained.