High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea (Cajanus cajan L.)

摘要

Epicotyl, hypocotyl, leaf, cotyledon and cotyledonary nodal segment explants from 7-day-old seedlings of Cajanus cajan cv. Hyderabad C were cultured on MS medium augmented with different concentrations of benzyladenine (BA) and kinetin. Among the various concentrations tested, 2.0 mg/litre BA or kinetin was best for maximum shoot bud differentiation. Percentage and number of shoots per explant showing differentiation of shoot buds were higher on MS medium supplemented with BA than with kinetin. Elongation of multiple shoots was obtained on MS medium fortified with BA in combination with NAA and GA3. The optimal BA concentration for shoot elongation was 1.0 mg/litre. The combination of 1.0 mg/litre BA with 0.1 mg/litre NAA increased the number of multiple shoots as well as shoot elongation. Addition of GA3 along with BA + NAA markedly enhanced both multiple shoot proliferation and shoot elongation in all the explants. The elongated shoots were successfully rooted on MS medium containing differentauxins. Among them IBA at 0.2 mg/litre induced maximum frequency of rooting followed by NAA and IAA. Regenerated plants were successfully established in soil where 90-95% of them developed into morphologically normal and fertile plants.