Simultaneous Silencing of Five Lipoxygenase Genes Increases the Contents of alpha-Linolenic and Linoleic Acids in Tomato (Solanum lycopersicum L.) Fruits

摘要

alpha-Linolenic and linoleic acids are essential fatty acids (EFAs) for humans and required for maintenance of optimal health, but they cannot be synthesized by the human body and must be obtained from dietary sources. Using TomloxC fragment, TomloxD fragment, and partial TomloxA sequence that is highly identical with TomloxB and TomloxE, a RNAi expression vector was constructed. The construct was used to transform tomato cotyledon explants with the Agrobacterium-mediated co-cultivation method. The real-time reverse transcription polymerase chain reaction analysis showed that the expression of TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE in transgenic tomato plants was drastically repressed, which led to a marked decrease in the levels of lipoxygenase activity. Finally, higher accumulations of the endogenous a-linolenic and linoleic acids were detected in the transgenic tomato fruits, which were 1.65-3.99 and 2.91-4.98 times that of the non-transformed tomato fruits, respectively.