Comparative Metabolic Profiling Reveals Secondary Metabolites Correlated with Soybean Salt Tolerance

摘要

High-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry(HPLC-UV-ESI-MS)and HPLC-ESI-MS"analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds.Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties.Principle component analysis clearly demonstrated that it is possible to use secondary metabolites,for example,isoflavones and saponins,to discriminate between closely related soybean genotypes.Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance.These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.