Performance Characteristics of Countercurrent Separation in Analysis of Natural Products of Agricultural Significance

摘要

A standard test mix consisting of 21 commercially available natural products of agricultural significance,termed the GUESSmix,was employed to measure the countercurrent chromatography performance characteristics of a very popular quaternary solvent system family made up of hexane-ethyl acetate-methanol-water(HEMWat).The polarity range of the GUESSmix combined with the elution-extrusion countercurrent chromatography(EECCC)technique and the newly developed reciprocal symmetry(ReS)and reciprocal shifted symmetry(ReSS)plots allow liquid-liquid distribution ratios(K_D)to be plotted for every compound eluted on a scale of zero to infinity.It was demonstrated that 16 of the 21 GUESSmix compounds are found in the optimal range of resolution(0.25