Probing the Electronic Structure of Peptide Bonds Using Methyl Groups

摘要

The observed V_3 torsional barriers measured by microwave spectroscopy for nine methyl groups attached a to peptide bond linkages in five gas-phase biomimetics have been found to differ considerably from one molecule to the next and even depend on the position of substitution,being sensitive to structural changes at the other end of the peptide bond.In the search for an explanation for these results,ab initio calculations have been performed at the HF/6-311++G(d,p)level of theory and interpreted in terms of the natural bond orbitals and resonance structures of the peptide bond.These calculations reveal that resonance derealization in peptide bonds is influenced by methyl conformation through the coupling of vicinal sigma to sigma* orbital interactions with the n to pi*.Thus,CN double-bond character increases(and CO double-bond character decreases)as the methyl group is rotated from the syn to the anti position.A quasilinear correlation exists between the barriers to internal rotation of attached methyl groups and the relative importance of the two principal resonance structures that contribute to the peptide bond.