Determination of Prilocaine HCl in Pharmaceutical Preparations by GC-MS Method

摘要

The novel analytical method using gas chromatography with mass spectrometry detection (GC-MS) method for determination of prilocaine HCl in pharmaceutical preparations were developed and validated. The using lidocaine HCl as the internal standard (IS) was carried out on HP-5MS capillary column. The calibration curves were linear (R = 0.999) in concentration range of 40-1000 ng/mL of prilocaine HCl. The LOD and LOQ values were 15 and 31 ng/mL, respectively. The within- and between-day RSD and RE % values of proposed method were found to be < 3.4 and < 3.5 %. respectively. o-Toluidine was formed in stored on auto sampler during 72 h was determined by proposed method. The developed method can be directly and easily applied for determination of prilocaine HCl without derivatized in pharmaceutical preparations using internal standard methodology.