Determination of DL-Methionine with Electro-chemiluminescence Molecularly Imprinting Method

摘要

In order to establish a novel EC-MIP method in the determination of DL-methionine in human urine, a carbon electrode of [Ru(bpy)3]~(2+) was modified with the molecular imprinting polymers electro-deposited with the sol-gel method. The abilities of the electrochemical and electro-chemiluminescence characteristics of the above electrode were then investigated with the quantitative determination of dL-methionine in human urine. The method exhibited the high sensitivity and excellent selectivity towards DL-methionine. With the optimized conditions. the linear range of dL-methionine was from 1 × 10~(-11) mol/L to 1 × 10~(-8) mol/L, with the LOD as 2.6 × 10~(-12) mol/L. This method could be successfully applied to the determination of dL-methionine in human urine samples for the precaution of certain diseases.