Simultaneous Determination of Four Classes of Acanthopanax giraldii Hormone by HPLC Method

摘要

A simple and quick method is described for the confirmation of hormones in annual branches of Acanthopanax giraldii. The hormones in the samples were extracted by using methanol and analyzed by HPLC. Chromatography separation was performed by using Agilent 1100 series HPLC system with symmetry C_(18) column and gradient elution with a mixture of two solvents: methanol and 1 % aqueous acetic acid. The effluent was monitored by a VWD detector set at 254 nm. The average recoveries of zeatin, gibberellins, indole acetic acid and abscisic acid were 99.43, 97.64, 93.16 and 95.28, respectively (n = 3). The limits of detection and quantification were 0.044 and 0.133 μg mL~(-1). respectively. The method has been successfully applied to analysis of 64 samples, zeatin, gibberellins, indole acetic acid and abscisic acid content of leaves ranged from 3.57-84.83, 0.00-43.09, 0.00-92.51 and 0.00-53.78 ng g~(-1), the zeatin, gibberellins, indole acetic acid and abscisic acid content of rinds ranged from 3.71-38.91, 0.00-34.36, 0.00-6.57 and 0.00-22.07 ng g~(-1).