Determination of Synergistic Effects of Catalase and Natural Antioxidants in Scavenging ~·OH by Capillary Electrophoresis

摘要

A capillary electrophoresis method for the determination of serum ~·OH is established. The ascorbic acid/Fe~(2+) system was used to generate the ~·OH and the benzoic acid was used to trap the ~·OH. The optimal conditions such as the SDS concentration, buffer pH and separation voltage were investigated under the optimal condition, the impacts of substrate concentrations and reaction time towards the production of ~·OH were studied. All the substances could be well separated basically; the linear range of the reaction product (m-hydroxylbenzoic acid) was 5 × 106to 3 × 10"3mol/L and the limit of detection was 1.0 × 10~(-6) mol/L. Then the method was applied to determine the IC_(50) of catalase, catechin and quercetin towards the serum ~·OH. The method was stable and quick and could be specifically applied into the research of the synergistic effects of catalase and natural antioxidants in scavenging the serum ~·OH.