Spectrophotometric Determination of Protein with DBC-Chlorophosphonazo

摘要

A new spectrophotometric method for the determination of protein has been developed using the property that bovine serum albumin and DBC-chlorophosphonazo form a compound. The maximum absorption wavelength of the compound is 606 nm. Beer's law is obeyed over the range of 25-250 μ /mL of bovine serum albumin contents. The detection limit of this method is 9.52 μ g/mL. Protein content in human serum has been successfully determined.