A method to improve the induction of callus from saffron ( Crocus sativus Linneo) corms.

摘要

In vitro callus and suspended cells induction's response was compared from saffron corms (Crocus sativus L.) from a Spanish cultivar; some corms were immediately cultivated in vitro in November 2006 and the others were preserved in distilled water in laboratory until daughter corms formed and in vitro cultured in May 2007. For the explants of the mother corms, the medium used was Murashige-Skoog (MS), with 2-4 dichlorophenoxy acetic acid (2,4-D, 0, 1, 2 and 4 mg.L-1) and kinetin (Kin., 0, 1 mg.L-1) in liquid medium and in agar gelified medium. The explants of daughter corms had the same treatments and they were planted in MS medium with Naphtalene Acetic Acid (NAA, 0, 1, 2 and 4 mg.L-1) and Kin (0, 1 mg.L-1), as well in gel as liquid media. In mother corms in gel the average induction time was 31.6 days with 24% induction frequency and 85% contamination rate; in liquid medium the contamination reached 100%. In the case of daughter corms obtained in laboratory, treatments in gel had an average induction time of 12.4 days and 100% induction frequency and 15% contamination; in liquid treatments the appearance time of free cells varied from 10 to 14 days with 100% induction frequency and 25% contamination rate; there was no significant difference (P