Use of yeasts as a bioindicator for the ultraviolet radiation action on living organisms

摘要

The paper presents the results of the comparative research on the fungicidal (lethal) effect of the exposure of a Saccharomyces cerevisiae baker's yeast culture to UV radiation and the reduction of this effect by photoreactivation with visible light of the UV irradiated culture. For the cultivation of control and irradiated yeasts as well as for the numbering of the living cells/mL (TGN), there have been used the standard methods. The ultraviolet radiation has a differentiated fungicidal effect on Saccharomyces cerevisiae bake yeast cells, depending on the initial concentration of the yeast cells and the irradiation time. The changes the yeasts under the action of UV radiations are variable and concern the shape, dimensions, celulare structure and the type of the culture. Thus, there is possible to use the Saccharomyces cerevisiae yeast as a simple and efficient bioindicator of the UV radiation effect on living organisms.