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首页> 外文期刊>Nuclearmedicine >Multiple cardiac masses and distant metastatic foci in a patient with high grade pleomorphic sarcoma of the heart revealed by follow-up FDG PET/CT
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Multiple cardiac masses and distant metastatic foci in a patient with high grade pleomorphic sarcoma of the heart revealed by follow-up FDG PET/CT

机译:随访FDG PET / CT显示心脏高度多形性肉瘤患者出现多发性心脏包块和远处转移灶

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摘要

Human semen is a complex biological matrix. It contains mature spermatozoa, immature germ cells, residual apoptotic bodies and, in some cases, epithelial cells and leucocytes. Hence, one of the challenges in applying flow cytometry in spermatology is the correct recognition of spermatozoa and their separation from signals of other semen cells/elements. In this study, we show that semen spermatozoa are included in a well-defined, flame-shaped FSC/SSC region (FR), by demonstrating that the count of the spermatozoa contained in such region overlaps that obtained by microscopy in the same samples. In FR, nuclear staining of semen samples reveals three different populations: unstained, brighter and dimmer. Unstained elements were previously characterized as apoptotic bodies of testis origin and the brighter elements represent the majority of semen spermatozoa, whereas the composition and the origin of the population with a lower nuclear staining is less clear, albeit we have previously shown that all the elements constituting it are positive for TUNEL. In this study, we sorted all the elements contained in FR region and demonstrated that the dimmer elements are spermatozoa. To further characterize dimmer spermatozoa, we evaluated apoptotic caspases and chromatin immaturity, the latter detected by aniline blue (AB) and chromomycin A (CMA3) staining. We found that caspases were much more expressed in the dimmer spermatozoa (71.4 ± 18.8%) than in the brighter (46.7 ± 15.1%), whereas similar amounts of spermatozoa with chromatin immaturity were found in both populations (brighter, AB: 48.2 ± 19.5%; CMA3: 48.5 ± 20.4% and dimmer, AB: 43.4 ± 19.8%; CMA3: 36.1 ± 18.0%). Hence, the role of apoptosis in generating dimmer spermatozoa and their DNA fragmentation appears clear, whereas the involvement of defects during the chromatin packaging remains elusive.
机译:人精液是一种复杂的生物基质。它包含成熟的精子,未成熟的生殖细胞,残留的凋亡小体,在某些情况下还包括上皮细胞和白细胞。因此,将流式细胞术应用于精子学的挑战之一是对精子的正确识别及其与其他精液细胞/元素信号的分离。在这项研究中,我们证明精子的精子数量包含在一个明确定义的火焰状FSC / SSC区域(FR)中,这表明该区域所含的精子数量与通过显微镜检查获得的精子数量相同。在FR中,精液样品的核染色揭示了三个不同的种群:未染色,更亮和更暗。以前未染色的元素被表征为睾丸起源的凋亡小体,亮的元素代表了精子的大部分,而核染色较低的人群的组成和起源则不清楚,尽管我们先前已经证明了构成精子的所有元素。对TUNEL来说是积极的。在这项研究中,我们对FR区域中包含的所有元素进行了分类,并证明了较暗的元素是精子。为了进一步表征较暗的精子,我们评估了凋亡的半胱氨酸蛋白酶和染色质不成熟,后者通过苯胺蓝(AB)和染色霉素A(CMA3)染色检测。我们发现半胱氨酸蛋白酶在较暗的精子中表达更高(71.4±18.8%),而在较亮的精子中表达更高(46.7±15.1%),而两个群体中染色质不成熟的精子数量相似(更亮,AB:48.2±19.5) %; CMA3:48.5±20.4%和较暗,AB:43.4±19.8%; CMA3:36.1±18.0%。因此,细胞凋亡在产生较暗的精子及其DNA片段化中的作用似乎是清楚的,而在染色质包装过程中缺陷的参与仍然难以捉摸。

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