Evaluation of FTA? Paper for Storage of Oral Meta-Genomic DNA

摘要

Aim: The purpose of the present study was to evaluate the short-term storage of meta-genomic DNA from native oral biofilms on FTA? paper. Materials and Methods: Thirteen volunteers of both sexes received an acrylic splint for intraoral biofilm formation over a period of 48 hours. The biofilms were collected, resuspended in phosphate-buffered saline, and either stored on FTA? paper or directly processed by standard laboratory DNA extraction. The nucleic acid extraction efficiencies were evaluated by 16S rDNA targeted SSCP fingerprinting. The acquired banding pattern of FTA-derived meta-genomic DNA was compared to a standard DNA preparation protocol. Sensitivity and positive predictive values were calculated. Results: The volunteers showed inter-individual differences in their bacterial species composition. A total of 200 bands were found for both methods and 85% of the banding patterns were equal, representing a sensitivity of 0.941 and a false-negative predictive value of 0.059. Conclusion: Meta-genomic DNA sampling, extraction, and adhesion using FTA? paper is a reliable method for storage of microbial DNA for a short period of time.