SENSITIVE AND EFFICIENT DETERMINATION OF GABAPENTIN IN HUMAN SERUM BASED ON CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION

摘要

A sensitive and efficient analytical method for gabapentin (GBP) in human serum based on capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection has been established. 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein (SAMF), a new synthesized fluorescent reagent, was used for precolumn derivatization of the non-fluorescent drag in serum. y-Aminobutyric acid (GABA) was used as an internal standard (I.S.). The best derivative condition was obtained in phosphate buffer (pH 8) at room temperature for 10 min. Optimal separation and detection were obtained with a background electrolyte (BGE) of 3.5 x 10~(-2) M phosphate buffer (pH 5.5) and laser-induced fluorescence detection excited at 473 nm. The method developed for GBP was linear over the concentration range of 4.0 x 10~(-9) to 4.0 x 10~(-7) M. The concentration limit of detection was 2.0 x 10~(-10) M (signal-to-noise ratio = 3). The sensitive method was used for the determination of GBP in serum samples.