Sensitive and rapid determination of nitric oxide in human serum using microchip capillary electrophoresis with laser-induced fluorescence detection

摘要

Microfluidic chip capillary electrophoresis with laser-induced fluorescence detection is employed for direct determination of trace nitric oxide in human blood using diaminorhodamines as the fluorescence probe. Factors influencing the separation and detection processes were systematically studied. Complete and fast separation of the highly fluorescent triazole formed was achieved within 45 s, and the relative standard deviations values of migration time and peak area were less than 3%. The detection limit of NO was 3.0 nmol.L-1 (at a signal-to-noise ratio of 3) and the liner range was from 1.0 × 10-8 mol.L-1 to 3.0 × 10-6 mol.L-1. The method has been applied to the determination of NO in serum of healthy persons and patients suffering from diseases, with recoveries varying from 92.65 to 98.43%. Keywords Nitric oxide - DAR-4M - Microchip capillary electrophoresis - Laser-induced fluorescence detection