ESMS and NMR investigations on the interaction of the anticancer drug cisplatin and chemopreventive agent selenomethionine

摘要

The resctions of L-selenomethionine (L-Se-MetH) with cisplatin, cis-[PtCl_2(NH_3)_2], were investigated using electrospray mass spectroscoy (ESMS) and 2-D [~1H-~(15)N] NMR spectroscopy. The reaction intermediates and products identified were cis-[PtCl(L-Se-MetH)(NH_3)_2]~+, cis-[Pt(L-Se-Met)(NH_3)_2]~+, [PtCl(L-Se-MetH)(NH_3)]~+ and [Pt(L-Se-Met)(L-Se-MetH)]~+. Some binuclear adducts were also detected by ESMS during the reaction, although they existed inminor amounts. Similar reactions with L-MetH were also conducted under similar conditions for comparison. This work provides the first detailed studies on the reactionof a platinum-based drug with L-Se-MetH