The enhancement by means of magnesium salt of the production of a thermal stable nuclease of Vibrio vulnificus with a recombinant Escherichia coli was investigated. Supplementation of the medium with a proper amount of magnesium salt was found to be critical for enzyme production. Cultivation of the recombinant strain in LB medium resulted in a nuclease yield of 62 U/mL. Supplementation of the LB medium with 50 mM MgCl2 increased the yield to 380 U/mL. Increasing the cell concentration could enhance the nuclease yield provided that a sufficient amount of magnesium ion was present in the medium. However, an overdose of magnesium salt had an adverse effect on enzyme production, where the type of magnesium salt was an influential factor. A nuclease yield of 3750 U/mL was achieved by supplementing LB medium with additional carbon and nitrogen sources, and with a proper concentration of MgSO4. [References: 12]
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机译：研究了通过镁盐增强用重组大肠杆菌生产热稳定性弧菌核酸酶的方法。发现向培养基中添加适量的镁盐对于酶的生产至关重要。在LB培养基中培养重组菌株导致核酸酶产率为62U / mL。用50 mM MgCl2补充LB培养基可使产量增加到380 U / mL。如果培养基中存在足够量的镁离子，则增加细胞浓度可以提高核酸酶的产量。然而，过量的镁盐对酶的产生有不利影响，其中镁盐的类型是影响因素。通过用额外的碳和氮源以及适当浓度的MgSO4补充LB培养基，可实现3750 U / mL的核酸酶产率。 [参考：12]
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