QUALITATIVE AND QUANTITATIVE ANALYSIS OF 15 ACTIVE CONSTITUENTS IN JIWEILING FREEZE-DRIED POWDER BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

摘要

Qualitative characterization and quantitative analysis of 15 bioaclive constituents in Jiweiling freeze-dried powder (JWL) have been achieved by reversed-fhase high performance liquid chromalography coupled with tandem mass speclromelry, including five flavonoids and ten ginse-nosides. Chromalographic separation was performed on an Agilent ZORBAX Eclipse XDB-Cig HPLC column, with gradient elution of 0.1% formic acid aqueous solution and acelonilrile as mobile phase. The compounds were delected in the negative ion mode with multiple reaction monitoring (MRM) using a triple quadrupole mass spectrotneler equipped with an eleclrospray ioniza-lion interface. By using of this method, 15 bioaclive components were unequivocally identified on the basis of their characteristic fragmentation patterns. All calibration curves showed good linearity (r2 > 0.998) within the test ranges. The LOD, LOQ specificity, precision, and accuracy for the method were validated. The proposed method was successfully applied to analyze the quality of 12 batches of JWL. The results indicated that this analytical method was simple and suitable for the identification and quality assessment of JWL.