RAPID SCREENING OF OFLOXACIN ENANTIOMERS IN HUMAN URINE BY MOLECULARLY IMPRINTED SOLID-PHASE EXTRACTION COUPLED WITH LIGAND EXCHANGE CHROMATOGRAPHY

摘要

A simple molecularly imprinted solid-phase extraction coupled with ligand exchange chromalogmpliy method was developed for rapid screening of R-(+)- and S-(-)-ofloxaxin in human urine. New molecularly imprinted microspheres (MIMs) were synthesized by aqueous suspension polymerization using pazufloxacin as pseudo template and it showed high affinity and selectivity to ofloxacin in aqueous solution. Baseline separation of ofloxacin enanliomers in human urine were obtained on a C_(18) column in less than 20 mm and no interferences from the protein or endogenous compounds were observed. Under the optimal conditions, good linearity of the method was obtained in the range of 0.07 to 60 mug/mL with the correlation coefficient of r~2 > 0.9996 and the relative standard deviation of 2.9-4.5 %. The detection, limits signal-to-noise ratio of 3 (S/N=3) was 0.03 mug/mL and 0.04 mug/mL for S-(-)- and R-(+)- ofloxaxin, respectively. The proposed method could be potential applied to investigate the slereoselectivity and pharmacokinet-ics of ofloxacin enantiomers in human body.