Immunopathological and molecular approaches for the diagnosis of Salmonellosis in natural cases of bovine abortion.

摘要

Rapid and precise diagnosis in natural field cases of bovine abortion warrants use of most sensitive and reliable diagnostic methods. In the present study, histopathological, immuno-histochemical and PCR technique(s) have been applied for diagnosis of abortion cases due to Salmonellosis. Histopathologically, multifocal lymphoid granulomas were observed in liver of aborted fetuse(s) which showed positive immuno-reactivity to the anti-Salmonella polyclonal antibody. PCR for diagnosis of salmonellosis in the aborted bovine fetuses found two positive samples out of the total 113 samples tested. Primers S18/S19 pair amplified a 159 bp region of the sequence encoding an ompC gene of Salmonella species.