Characterization of interaction between protein and carbohydrate using CZE.

摘要

The present study describes the application of the capillary zone electrophoresis (CZE) to investigate interactions between Concanavalin A (Con A) and the one of their major components (L-asparagin) with a variety of carbohydrates (D-glucose, D-fructose, D-mannose D-galactose, maltose and lactose). It was observed that the carbohydrates show different interactions with Con A and L-asparagin. Addition of carbohydrates to the electrolyte buffer and different concentrations of carbohydrates led to a change of the migration time and the ionic mobility of Con A and L-asparagin. A mathematical model for quantitative evaluation to calculate the aggregation constants (K) was used. Our investigations show that it is possible to characterize the interaction between carbohydrates and proteins or amino acid quantitatively using CZE.