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首页> 外文期刊>Journal of Microscopy >Estimation of subcellular organelle volume from ultrathin sections through centrioles with a discretized version of the vertical rotator.
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Estimation of subcellular organelle volume from ultrathin sections through centrioles with a discretized version of the vertical rotator.

机译:使用离散化版本的垂直旋转器,估计从超薄切片到中心粒的亚细胞器的体积。

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摘要

A method for the fast and efficient estimation of the volume (but not surface area) of subcellular organelles is presented. It consists of a rotator/coaxial-section approach based on the Pappus theorem and represents a discretized version of the vertical rotator where, instead of measuring intercept lengths, the points in distance classes are counted. Centrioles serve as a unique reference 'double-point' with constant size allowing unbiased cell selection from the whole population with equal probability and without the disector application. The sandwich-like method of sample preparation allows comparison of control and experimental cases with the same errors caused by overlapping and overprojection. Test experiments demonstrated that the vertical discretized rotator was an efficient and precise tool for the estimation of volume and that a few independent sections of unknown thickness were sufficient for the quantification of one experimental point.
机译:提出了一种快速有效地估计亚细胞器细胞体积(而不是表面积)的方法。它由基于帕普斯定理的旋转器/同轴截面方法组成,代表了垂直旋转器的离散化版本,在该版本中,不是测量截距,而是计算距离类中的点。质心充当具有恒定大小的唯一参考“双点”,允许从整个群体中以相同的概率无偏见地选择细胞,而无需使用解剖器。样品制备的三明治式方法可以比较对照样品和实验样品,它们具有由重叠和过度投影引起的相同误差。测试实验表明,垂直离散旋转器是一种高效,精确的体积估算工具,一些厚度未知的独立部分足以量化一个实验点。

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