Partial hydrolysis of soybean oil by phospholipase A1 to produce diacylglycerol-enriched oil.

摘要

Partial hydrolysis of soybean oil catalysed by phospholipase A1 (Lecitase Ultra) in a solvent-free system was carried out to produce a diacylglycerol (DAG)-enriched soybean oil. 5 reaction parameters of partial hydrolysis were investigated. The upper oil layer of the reaction mixture was molecularly distilled at 150 degrees C to yield a DAG-enriched oil with 42.64 (wt.%) of DAG. DAG-enriched oil was distilled a 2nd time at 250 degrees C to yield a DAG oil with 78.68 (wt.%) of DAG. The composition of acylglycerols in the DAG-enriched soybean oil was determined by HPLC and HPLC/electrospray ionization/MS. The released fatty acids from the partial phospholipase A1 hydrolysis of soybean oil showed a higher saturated fatty acid content than that of the raw material. Compared with other lipase-catalysed processes, this new phospholipase A1 preparation has the advantage of reducing production of monoacylglycerol by-product. Compared with the lipase-catalysed process, this new phospholipase A1 shows the advantage of low cost and low by-product monoacylglycerol production. The new process has a potential application in the commercial production of DAG oil.