Modelling the growth and bacteriocin production by Lactobacillusamylovorus DCE 471 in batch cultivation

摘要

A model was set up to describe the production of amylovorin L471 by Lactobacillus amylovorus DCE 471, on a laboratory scale, in which the cells are grown in MRS (deMau-Rogosa-Sharpe) broth. The main features of the dynamic model are: (i) increase of the biomass according to a logistic equation; (ii) non-growth-associated consumption of substrate (maintenance metabolism); and (iii) primary metabolite kinetics for the bacteriocin production. The main purpose was to set up a simple empirical model to examine growth and bacteriocin production in different conditions. Parameters estimated from a fermentation with 20 g l(-1) glucose (w/v) could be used to predict the evolution of cell dry mass, glucose and lactic acid concentration of fermentations, performed with 5, 30, 40 and 60 g l(-1) initial glucose. The influence of the operating temperature (30, 37 and 45 degrees C) on the model parameters was also investigated. The proposed model was able to describe growth and bacteriocin production in all cases. The specific bacteriocin production rate was found to vary strongly with temperature, with 30 degrees C as the best value. Variation of the operating temperature from 37 to 30 degrees C appeared to significantly enhance the specific bacteriocin production.