Synthesis, characterization, and use of 2-[(~2H_9)butoxy] acetic acid and 2-(3-methylbutoxy) acetic acid as a internal standard and an instrument performance surrogate, respectively, for the gas chromatographic-mass spectrometric determination of 2-b

摘要

2-[(~2H_9)Butoxy]acetic acid and 2-(3-methylbutoxy) acetic acid were synthesized, mixed with 2-butoxyacetic acid, and separated by capillary gas chromatography on a fused-silica column with a length of 50 m, inside diameter of 0.200 mm, and a "free fatty acid phase" wall coating of 0.3 μm film. 2-[(~2H_9)Butoxy]acetic acid, 2-butoxyacetic acid, and 2-(3-methylbutoxy) acetic acid were baseline resolved at retention times of 13.55,13.78, and 15.20 min; 2-(3-methylbutoxy) acetic acid having a peak efficiency of 360 000. Mass spectrometric detection using selected ion monitoring at m/z 66, 57, and 71 showed linear analytical responses from 0.04 ng to at least 200 ng with a limit of detection of 0.04 ng for 2-butoxyacetic acid.