Detection of D-serine in rat brain by capillary electrophoresis with laser induced fluorescence detection

摘要

A capillary zone electrophoresis method with laser induced fluorescence detection for the chiral separation of highly fluorescent enantiomeric derivatives of D/L-Serine from 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-D/L-Serine) was developed and optimized. Enantiomeric separation of NBD-D/L-Serine was accomplished by using 40 mM hydroxypropyl-p-cyclodextrin (HP-P-CD) contained in 100 mM borate buffer, pH 10.0. A 70 cm (effective length of 50 cm) uncoated fused-silica capillary at a voltage of 15 kV was used for the separation. The optimized electrophoretic conditions were subsequently applied to the analysis Of D-Serine in rat brain, and satisfactory analytical results with respect to accuracy were obtained. This assay showed acceptable precision, with linearity in the D-Serine concentration range of 0.2-20.0 mu M. The limit of detection for D-Serine was 3.0 x 10(-7) M. (c) 2005 Elsevier B.V. All rights reserved.