Preparation and evaluation of a molecularly imprinted sol-gel material for on-line solid-phase extraction coupled with high performance liquid chromatography for the determination of trace pentachlorophenol in water samples

摘要

A highly selective imprinted amino-functionalized silica gel sorbent was prepared by combining a surface molecular imprinting technique with a sol-gel process for on-line solid-phase extraction-HPLC determination of trace pentachlorophenol (PCP) in water samples. The PCP-imprinted amino-functionalized silica sorbent was characterized by FT-IR, SEM, nitrogen adsorption and the static adsorption experiments. The imprinted functionalized silica gel sorbent exhibited high selectivity and offered a fast kinetics for the adsorption and desorption of PCP. The prepared sorbent was shown to be promising for on-line solid-phase extraction for HPLC determination of trace levels of PCP in environmental samples. With a sample loading flow rate of 5 ml min(-1) for 2 min, an enhancement factor of 670 and a detection limit (S/N = 3) of 6 ng 1(-1) were achieved at a sample throughput of five samples h(-1). The precision (RSD) for nine replicate on-line sorbent extractions of 10 mu g 1(-1) PCP was 3.8%. The sorbent also offered good linearity (r = 0.9997) for on-line solid-phase extraction of trace levels of PCP. The method was applied to the determination of PCP in local lake water, river water and wastewater samples. (c) 2005 Elsevier B.V. All rights reserved.