Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Vizcaino-Caston Isaac; Kelly Catherine A.; Fitzgerald Annabel V. L.; Leeke Gary A.; Jenkins Mike; Overton Tim W.

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Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

机译：开发一种从细菌中分离多羟基链烷酸酯以进行筛选研究的快速方法

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We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.

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《Journal of Bioscience and Bioengineering》 |2016年第1期|共4页
作者
Vizcaino-Caston Isaac; Kelly Catherine A.; Fitzgerald Annabel V. L.; Leeke Gary A.; Jenkins Mike; Overton Tim W.;
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正文语种 eng
中图分类生物化学;生物工程学（生物技术）;酿造工业;
关键词
Polyhydroxyalkanoate; Dimethyl sulphoxide; Solvent extraction; Bioplastic;

机译：聚羟基链烷酸酯;二甲基亚砜;溶剂萃取;生物塑料;

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1. Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies [J] . Vizcaino-Caston Isaac, Kelly Catherine A., Fitzgerald Annabel V. L., Journal of Bioscience and Bioengineering . 2016,第1期

机译：开发一种从细菌中分离多羟基链烷酸酯以进行筛选研究的快速方法
2. Methods for rapid screening and isolation of bacteria producing acidic lipase: feasibility studies and novel activity assay protocols [J] . Liu Chien-Hung, Chen Wen-Ming, Chang Jo-Shu World Journal of Microbiology & Biotechnology . 2007,第5期

机译：快速筛选和分离产生酸性脂肪酶细菌的方法：可行性研究和新的活性测定方案
3. Methods for rapid screening and isolation of bacteria producing acidic lipase: feasibility studies and novel activity assay protocols [J] . Chien-Hung Liu, Wen-Ming Chen, Jo-Shu Chang World Journal of Microbiology and Biotechnology . 2007,第5期

机译：快速筛选和分离产生酸性脂肪酶细菌的方法：可行性研究和新的活性测定方案
4. RAPID SCREENING METHODS TO ISOLATE PROLIFERATION RELATED COMPOUNDS FROM SUSPECT SAMPLES UTILIZING SOLID PHASE MICROEXTRACTION (SPME) TECHNOLOGY [C] . Armando Alcaraz, Brian D. Andresen, NATO NATO advanced research workshop on environmental aspects of converting CW facilities to peaceful purposes and derivative technologeis in modeling, medicine and monitoring . 2002

机译：利用固相微萃取（SPME）技术，从嫌疑样品中分离出增殖相关化合物的快速筛选方法
5. Molecular characterization and development of diagnostic methods for the rapid detection of Cercospora beticola isolates with resistance to fungicides. [D] . Obuya, James O. 2014

机译：分子特征和诊断方法的开发，可快速检测对杀真菌剂具有抗性的番茄锥孢菌。
6. A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater [O] . Mieko Higuchi-Takeuchi, Kumiko Morisaki, Keiji Numata -1

机译：从天然海水中分离产生聚羟基链烷酸酯的紫色非硫光合细菌的筛选方法
7. The bacterial identification and antimicrobial susceptibility testing are usually performed manually. Recently, the number of samples has increased and the need for automatification has grown. BioMerieux has investigated Vitek2 for this purpose. HUSLAB has assigned us to compare the identification and antimicrobial results with respect to routine methods and Vitek2. Furthermore, we evaluate test reactions, ESBL (Extended Spectrum Beta-Lactamase) production and the time what it takes to get results with Vitek2. In this study, we examined a total of 183 bacterial isolates including 76 ESBL-strains, 40 nonfermenting rods, 41 fastidious bacteria and 26 fresh clinical isolates. The results of routine methods were gathered from the patient database and following this, compared to the results given by Vitek2. Vitek2 correctly identified more than 96 f the ESBL-strains and fresh clinical isolates. The results of nonfermenting rods and fastidious bacteri a were 70 oncordant with those of the routine methods. Test reaction results of Api20E were 94 nd Api20NE 70 n concordance with Vitek2 results. Approximately 80 f all Vitek2 antimicrobial results and 90 f ESBL test results were correct. Compared to earlier studies, Vitek2 performed the tests faster than the routine methods. These results suggest that Vitek2 is capable of giving rapid and accurate results. However, nonfermenting rods and fastidious bacteria should be studied further. [O] . Järveläinen Marika, Roslund Iira, Silander Maarit 2006

机译：细菌鉴定和抗菌药敏试验通常是手动进行的。最近，样品的数量增加了，并且对自动化的需求也在增长。 BioMerieux为此进行了Vitek2的研究。 HUSLAB已指派我们比较常规方法和Vitek2的鉴定和抗菌结果。此外，我们还评估了测试反应，ESBL（扩展频谱β-内酰胺酶）的产生以及使用Vitek2获得结果所需的时间。在这项研究中，我们检查了总共183个细菌分离株，包括76个ESBL菌株，40个非发酵棒，41个营养细菌和26个新鲜临床分离株。从患者数据库中收集常规方法的结果，然后将其与Vitek2给出的结果进行比较。 Vitek2正确鉴定出超过96种ESBL菌株和新鲜的临床分离株。与常规方法相比，非发酵棒和精制细菌的结果为70。 Api20E的测试反应结果与Vitek2结果一致为94nd Api20NE 70 n。所有Vitek2抗菌结果约80 f和ESBL测试结果90 f正确。与早期的研究相比，Vitek2的测试速度快于常规方法。这些结果表明Vitek2能够给出快速而准确的结果。但是，应进一步研究非发酵棒和营养细菌。
8. Research and Development of Hazardous/Toxic Waste Analytical Screening Procedures. Available Field Methods for Rapid Screening of Hazardous Waste Materials at Waste Sites [R] . Snyder, R. E., Schulte, B. E., Mangoba, L., 1983

机译：危险/有毒废物分析筛选程序的研究与开发。在废物场地快速筛查危险废物的现场方法

Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

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