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Scientists genetically encode IR probe

机译:科学家对IR探针进行遗传编码

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摘要

Finding out how a protein interacts with other molecules in a cell can be challenging. Fluorophores such as rhodamine cannot label a specific protein. Although genetically encoded fluorophores such as GFP can identify a particular protein, they are often excited with UV radiation, a spectral region that frequently causes natural cellular components to also emit light. Therefore, mi-croscopists have to determine whether they are viewing their protein of interest or something else. In contrast, cells do not usually radiate when they are excited with IR light.
机译:找出蛋白质如何与细胞中的其他分子相互作用可能具有挑战性。荧光基团如罗丹明不能标记特定的蛋白质。尽管遗传编码的荧光团(例如GFP)可以识别特定的蛋白质,但它们通常会被紫外线辐射激发,该光谱区经常导致天然细胞成分也发光。因此,微观学家必须确定他们是否正在查看他们感兴趣的蛋白质或其他东西。相反,当细胞被红外光激发时通常不辐射。

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