In Vitro Investigation of Cell Compatibility of Pure p-TCP Granules

摘要

In this study,osteoblastic cells were isolated from rat bone marrow and characterized.The cells were cultured on p-TCP granules and the osteoblast/p-TCP constructs.For this purpose,bone marrow was harvested under sterile conditions.Cell aggregates were broken up by pipetting and a cell suspension was cultured in DMEM/F12.After three days,the cells that adhered to the surface of the flask were cultured in osteoblast medium.When the cells became confluent,they were passaged and cultured in 24-well polystyrene cell culture dishes.Characterization of the osteoblasts,cell proliferation and alkaline phosphatase activity were measured on days 1,7,14,21 and 30.To investigate the cell compatibility of the p-TCP granules,osteoblastic cells were cultured on p-TCP granules and a polystyrene cell culture dish (control group).Cell proliferation and alkaline phosphatase (ALP) activity were measured on days 1,7,14,21 and 30 in both groups.Cell growth significantly increased at each time point,but on day 30 a decrease was observed.The ALP activity also increased at each time point and also decreased on day 30.This study may be regarded as the first step leading to a therapy for various bone defects.