Proteolytic enzyme treatment: an improved method for elimination of egg stickiness in tench, Tinca tinca L., in aquaculture

摘要

The aim of the study was to improve the elimination of egg stickiness in tench as an attempt to increase the egg hatching rate. The usual desticking procedure involving treatment with milk solution/clay suspension resulted in a hatching rate of 74.1%. The highest hatching rate of 87.1% was found with a 10.0 ml L-1 enzyme treatment; hatching rates of 85.2 and 85.1% were recorded at 15.0 and 5.0 ml L-1 , but the hatching rate decreased to 80.0% at 20.0 ml L-1 . anova showed a significant effect of differing concentrations of enzyme and milk solution/clay suspension control (P < 0.0015, P < 0.0003) on the hatching rate and duration of egg incubation until hatching, respectively. Application of proteolytic alcalase enzyme, Merck EC 3.4.21.14, for desticking of tench eggs also decreased the time of egg handling from almost 1 h, necessary for traditional milk/clay desticking, to just 2 min.