New methods and tools for bioinorganic chemistry-Characterization of size and size distribution of nanoand micro-organic/inorganic materials: a comparison of scanning electron microscopy, dynamic light scattering, and flow field-flow fractionation with multiangle light scattering methods

摘要

Methods for the accurate determination of the size and size distribution of nano- and micro-sized organic/inorganic materials are essential for nano- and biotechnology. Among the methods available, flow field-flow fractionation with multiangle light scattering and UV absorption detectors (FFFF-MALS-UV) is considered to be a more effective method than field emission scanning electron microscopy (FE-SEM) and dynamic light scattering (DLS) for determining the size and size distribution of nanomaterials [1]. However, the raw values of size and size distribution obtained using these three methods are number-, volume-, and z-averaged, respectively. In order to compare the size and size distribution determined using different measurement methods, it is necessary to transform the raw values into the same dimensionality of length.