Determination of indoxacarb enantiomer residues in vegetables, fruits, and soil by high-performance liquid chromatography.

摘要

An effective chiral analytical method was developed for resolution and determination of enantiomers of the oxadiazine insecticide, indoxacarb, in cucumber, tomato, apple, pear and soil samples. Indoxacarb enantiomers were separated on a ChiralpakReg. AS-H column with n-hexane-ethanol (95 + 5, v/v) as the mobile phase. Validation involved complete resolution of each enantiomer, plus determination of linearity, precision, LOD and LOQ. The estimated LOD was 0.025-0.035 mg/kg, and the LOQ was 0.05 mg/kg for each indoxacarb enantiomer in different matrices. Average recoveries of the pesticide from all matrices ranged from 87.0 to 116.9% for fortification levels of 0.05, 0.1 and 5 mg/kg. Precision values associated with the analytical method, expressed as RSDr values, were <10.1% for the pesticide in all matrixes. It is suggested that this method could be used to evaluate environmental residues and the safety of agricultural products.