PEA SEEDLING EXTRACTS CATALYZE PROTEIN AMINE BINDING AND PROTEIN CROSS-LINKING .1. EVIDENCE FOR THE ROLE OF A DIAMINE OXIDASE

摘要

An enzymatic fraction (PPE) obtained by selective precipitation from a pea seedling extract was shown to catalyze putrescine incorporation. In order to elucidate the type of enzyme, transglutaminases or diamine oxidases (DAO), involved in this activity, the ability of this PPE fraction to catalyze their characteristic reactions, amine binding, deamidation, protein cross-linking, and oxidation was investigated. The use of specific enzyme inhibitors pointed out that only DAO was present in the extract and should be involved in the protein cross-linking reactions. This reaction was only catalyzed in the presence of diamines, especially putrescine and cadaverine, or polyamine like spermidine.