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Physical properties of egg white-dialdehyde starch films

机译:蛋清二醛淀粉薄膜的物理性质

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Films were cast from heated (40 degrees C for 20 min) alkaline (pH 11.25) aqueous solutions of egg white (EW) solids (9 g/100 mL of water), polyethylene glycol 400 (60% w/w of EW), yolk solids (10% w/w of EW), and dialdehyde starch (DAS) at 0, 2.5, 5, 7.5, or 10% (w/w) of EW. For all types of films, tensile strength (TS), percentage elongation at break (E), Hunter color values (L, a, and b), total soluble matter (TSM) after immersion in water at 25 degrees C for 24 h, and protein solubility (PS) after immersion for 12 h in buffers (pH 8) containing urea and urea/2-mercaptoethanol were determined. DAS addition increased (P < 0.05) film TS and yellowness (+b values) and reduced (P < 0.05) film TSM and PS in both buffer systems. These modifications in film properties suggested occurrence of cross-linking between EW protein and DAS. This was further supported by SDS-PAGE patterns. Such patterns for DAS-containing films revealed bands of aggregates, increasing in intensity with increasing amounts of DAS, which were absent from patterns of control EW films. [References: 70]
机译:从蛋清(EW)固体的碱性(pH 11.25)碱性水溶液(pH 11.25)(9 g / 100 mL水),聚乙二醇400(EW的60%w / w),蛋黄固体(EW的10%w / w)和二醛淀粉(DAS)分别为EW的0、2.5、5、7.5或10%(w / w)。对于所有类型的薄膜,将其在25摄氏度的水中浸泡24小时后的拉伸强度(TS),断裂伸长率(E),亨特色值(L,a和b),总可溶性物质(TSM),测定在含尿素和尿素/ 2-巯基乙醇的缓冲液(pH 8)中浸泡12小时后的蛋白质溶解度(PS)。在两个缓冲液系统中,添加DAS均会增加(P <0.05)薄膜TS和黄度(+ b值),并减少(P <0.05)薄膜TSM和PS。膜性质的这些改变表明在EW蛋白和DAS之间发生了交联。 SDS-PAGE模式进一步支持了这一点。含有DAS的薄膜的这种图案显示出聚集带,强度随DAS量的增加而增加,而对照EW薄膜的图案则没有。 [参考:70]

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