Reactive oxygen species activate the human elastin promoter in a transgenic model of cutaneous photoaging.

摘要

BACKGROUND: Generation of free radicals has been shown to play a role in cutaneous alterations resulting from ultraviolet radiation.OBJECTIVE: Cells from a previously described in vitro transgenic model of cutaneous photoaging were exposed to reactive oxygen species to determine if this results in elastin promoter activation.METHODS: Reactive oxygen species were generated using a hypoxanthine and xanthine oxidase system, and elastin promoter activation was measured using cells derived from transgenic mice containing the human elastin promoter.RESULTS: Free radical generation resulted in a greater than sixfold increase in elastin promoter activity, and this increase was blocked with the addition of catalase.CONCLUSION: Elastin promoter activation may play a role in the generation of solar elastosis in photoaged skin. Utilizing hypoxanthine and xanthine oxidase with the in vitro transgenic photoaging model results in a sensitive system for evaluating agents that may prevent oxidative damage.