Use of Cf-9 Gene-based Markers in Marker-assisted Selection to Screen Tomato Cultivars with Resistance to Cladosporium fulvum

摘要

Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for markerassisted selection (MAS). The Cf-9 locus confers resistance to most races of the leaf mold fungus Cladosporium fulvum and belongs to alarge gene family. We developed three gene-based markers (8F/10R, 8F/12R, and 10F/10R) from the Cf-9 gene sequence. Of the 101 tomato genotypes tested, 9 commercial F_1 hybrids carried 8F/10R-, 8F/12R-, and 10F/10R-specific bands. Our results demonstrate the usefulness of these markers for MAS for C. fulvum resistance.