Genetic Relationships of Some Pistacia Species Using RAPD and AFLP Maikers

摘要

Genetic diversity of plant species can be evaluated by various methods. The DNA markers are one of the most powerful techniques, because they are not affected by environmental factors as well as plant developmental stages. Markers such as RAPD, AFLP,RFLP and SSR are used for studying genetic diversity of plants. In order to corroborate our results and to minimize the experimental errors, in the present study, AFLP and RAPD markers were used. Genetic similarities (GS) were calculated for both markersusing the Jaccard and Dice algorithms. Six AFLP primer combinations used in this study generated 475 scorable bands, of which 336 bands were polymorphic, while the 12 RAPD primers produced 130 bands, of which 118 showed polymorphism. Using Jaccard and Dice algorithms, correlation between the genetic similarity and cophenetic coefficient (CCC) for RAPD marker were 0.82 and 0.77, respectively. In the case of AFLP, CCC was 0.91 and 0.89, respectively. The highest CCCs were calculated with the Jaccard method.