Effect of in vitro culture environments and culture methods on cormlet formation of Gladiolus.

摘要

Five gladiolus cultivars 'Adlib Scarlet', 'May Queen', 'Pacific Pink', 'Sharone' and 'White Race' were used in order to identify their specific culture environments and culture methods to obtain cormlets from a shoot base in vitro. There was a cultivar-by-medium-temperature interaction effect. The solid medium was appropriate only for cormlet formation of 'May Queen', while liquid medium was favored for the other four cultivars. The best culture environmol.mment for cormlets formation of 'Pacific Pink', 'Sharone' and 'White Race' was liquid medium treated at 20 degrees C. Fresh weight of cormlets of 'Adlib Scarlet' was highest when grown in liquid medium treated at 25 degrees C. There was no difference among the three temperatures for cormlet formation of 'May Queen' which grew best in solid medium. The suitable light intensity was 68 micro mol.m-2.s-1 PAR for 'May Queen', 'Sharone' and 'White Race', 34 micro mol.m-2.s-1 PAR for 'Adlib Scarlet' and 135 micro mol.m-2.s-1 PAR for 'Pacific Pink'. Increase in fresh weight was related to increased diameter regardless of different temperature, light intensity, media, or cultivar.