Biological nitrogen fixation in Casuarina equisetifolia using natural ~(15)N isotopic method

摘要

Casuarina equisetifolia seedlings, uninoculated or inoculated with Frankia strain Ce2 and Glomus fasciculatum were grown for 3 months in pots, harvested and N_2 fixation was measured using ~(15)N isotopic method. Maximum Ndfa percent was 83.3 when inoculated with Frankia and Vesicular Arbuscular Mycorrhiza at 10kg fertilizer N level. The modest amount of nitrogen fixed was attributed to the low soil fertility and the result of this experiment confirm that Frankia strain Ce 2 can be confidently recommended to inoculate Casuarinas along with Glomus fasciculatum in the field.