Comparison of white and red blood cell estimates in urine sediment with hemocytometer and automated counts in dogs and cats.

摘要

Background: Therapeutic decisions regarding urinalysis are commonly based on the presence of white and red blood cells. Traditionally, numbers per high-power field are estimated using wet-mount microscopic examination. This technique is not standardized and counts are likely prone to inaccuracy. In addition, differentiation of leukocyte types is not possible. Objectives: The aims of this study were to (1) compare WBC and RBC estimates using wet-mount examination with counts obtained using a hemocytometer, (2) assess if a hematology automated analyzer (Sysmex ST-2000iV/XT) provides reliable WBC and RBC counts in urine comparable to hemocytometer counts, and (3) evaluate air-dried Wright-Giemsa-stained urine drop sediment preparations for the determination of differential leukocyte counts. Methods: WBC and RBC counts were obtained by performing wet-mount estimates, manual hemocytometer counts, and Sysmex automated counts on 219 canine and feline urine samples. Results were correlated using Spearman rank correlation. Air-dried Wright-Giemsa stained sediment drop preparations (n=215) were examined for differential counts of leukocytes. Results: A low but significant association was found between WBC estimates on wet-mount examination and hemocytometer counts (rho=0.37, P<.01). There was a high and significant association when RBC counts were compared between wet-mount and hemocytometer evaluation (rho=0.7, P<.01). There was very high and significant interassay correlation between Sysmex data from duplicate samples for what the analyzer classified as WBC (rho=0.97, P<.01) and RBC (rho=0.94, P<.01). Low correlations were found between the Sysmex RBC counts and both wet-mount estimates and hemocytometer RBC counts (rho=0.43, P<.01 and rho=0.39, P<.01, respectively). Cell preservation in the air-dried sediment preparations was so poor that differential counts could not be performed. Conclusion: WBC and RBC estimates on wet-mount examination agreed with hemocytometer counts and are therefore considered adequate. The Sysmex ST-2000iV/XT did not provide reliable cell counts under the conditions used.Digital Object Identifier http://dx.doi.org/10.1111/vcp.12004