Micropropagation of Cochlospermum religiosum (L.) Alston. (Special Issue: Tree micropropagation and tissue culture.)

摘要

An in vitro micropropagation system has been developed for Cochlospermum religiosum (L.) Alston using node segments, obtained from 15-day-old aseptically grown seedlings, cultured on MS medium containing 6-benzyladenine (BA) and kinetin (Kn) either alone, or in combination. The regeneration medium with combined BA (4.44 micro M) and Kn (4.65 micro M) showed highest mean number of shoots (3.01+or-0.32), increased shoot length (1.21+or-0.06), and 80% regeneration response within 21 days. To initiate subculture, when separated and transferred to fresh medium with different levels of BA (4.44, 6.66 and 8.87 micro M) and Kn (4.65, 6.97 and 9.29 micro M), the medium containing BA (8.87 micro M) and Kn (4.65 micro M) resulted in further elongation of regenerated shoots. This phytohormone combination caused an increase in shoot length (2.23+or-0.05) within 42 days from the beginning of culture. The regenerated shoots showed optimal rooting response (80%) on medium containing half strength MS salts, 0.8% agar and 4.90 micro M indole-3-butyric acid (IBA). The plantlets established in vitro were transferred to pots containing sterilized soil and vermiculite (1:1) mixture. On transfer to the greenhouse, 65-70% plants survived. The present investigation for the first time describes an efficient micropropagation protocol for C. religiosum.