Identifying Differently Expressed Transcripts between a Novel Late-Ripening Citrus sinensis Mutant and its Wild Variety during Fruit Ripening using cDNA-AFLP

摘要

'Fengjiewancheng' (FJWC) is a late-ripening bud sport of 'Fengjie72-1' navel orange (FJ72-1). To investigate transcript differences during fruit ripening between the two cultivars and to get clues as to the mechanisms monitoring the late-ripening traits, we compared their transcript profiles during fruit ripening using cDNA-AFLP. As a result, 144 transcript-derived fragments (TDFs) were found differently expressed in FJWC compared with FJ72-1 of which 129 TDFs were recovered and assembled into 51 uniTDFs. Blast annotation indicated that 39 uniTDFs were assigned with putative function in signal transduction, control of gene expression and carbohydrate biosynthesis. Some TDFs shared relatively high identities with known functional genes and showed differential expression patterns in fruit pulp or peel between FJWC and FJ72-1 during fruit ripening, implying a potential role in the formation of the late-ripening phenotype.