Transvaginal ultrasound-guided oocyte retrieval following FSH stimulation of domestic goats.

摘要

In the first of 2 experiments, 56 goats were assigned to one of 4 ovarian stimulation treatments during the autumn breeding season. The treatments differed in the type of FSH (porcine or equine) and the dose of FSH (20 or 40 mg total dose). For eachof the 4 treatments, oocytes were recovered by laparoscopic or transvaginal ultrasound-guided aspiration procedures. Parameters evaluated were the number of follicles per ovary and oocyte recovery. There was no difference between the 2 sources of FSH for either of the parameters. For the 2 FSH doses combined over sources, there was no difference in the number of follicles detected per animal (12.6 and 14.4 for the 20 and 40 mg doses respectively), but the number of oocytes recovered was higher for the40 mg dose (12.4 vs. 6.9; P<0.05). For the laparoscopic and transvaginal ultrasound methods, the number of follicles detected averaged 17.4 and 9.5 respectively (P<0.05), and the number of oocytes recovered 14.4 and 4.3 (P<0.05). The second experiment was carried out in the non-breeding season. 16 animals that had not been aspirated previously and 16 animals that had been aspirated previously were placed on 2 ovarian stimulation treatments and the 2 aspiration procedures as in the first experiment in a2 X 2 X 2 factorial design. The ovarian stimulation treatments were as follows: (1) implanted with norgestomet and FSH given daily for 4 days starting 10 days after implantation; (2) treated as in (1) but FSH given 3 days after implantation and implantskept in place until aspiration. None of the treatment effects was significant except for a higher oocyte recovery rate for laparoscopic compared with transvaginal aspiration (11.0 vs. 4.3; P<0.05). Unlike animals subjected to repeated aspiration by laparoscopy, there was no evidence of adhesions in animals subjected to transvaginal aspiration.