Limited Sampling Strategy of Partial Area Under the Concentration-Time Curves to Estimate Midazolam Systemic Clearance for Cytochrome P450 3A Phenotyping

摘要

Objective: Intravenous (IV) midazolam is the preferred cytochrome P450 (CYP) 3A probe for phenotyping, with systemic clearance (CL) estimating hepatic CYP3A activity. A limited sampling strategy was conducted to determine whether partial area under the concentration-time curves (AUCs) could reliably estimate midazolam systemic CL during conditions of CYP3A baseline activity, inhibition, and induction/activation.