Expression of Sry genes transfected to a cultured mouse Sertoli cell line, TM-4, induces the P450 aromatase gene expression

摘要

In mammals, SR Y (the sex determining region on the Y; Sry in the mouse) gene located at the TDF (testis determining factor; Tdy in the mouse) locus, plays a key role in the sex determination. In the mouse, Sry open reading frame (ORF) is flanked at both 5' and 3' ends with a large inverted repeat, causing the formation of untranslatable circular transcripts (Capel et al., 1993). The expression of linear transcripts are developmentally regulated in this species and takes place transiently in the genital ridge between 10.5 and 12.5 day post coitum (Koopman et al., 1990). In the past attempts reported in the literature to examine the effects of expression of Sry exogenously introduced into cultured cells, the transfected Sry generated only the untranslatable circular transcript's (Capel et al, 1993).