Chiral resolution of cypermethrin on cellulose-tris(3,5-dimethylphenyl-carbamate) chiral stationary phase

摘要

Cellulose-tris(3,5-dimethylphenylcarbamate)was synthesized and coated on aminopropylsilica to prepare a chiral stationary phase. Cypermethrin, alpha-, theta-and beta-cypermethrin were well resolved by high-performance liquid chromatography on this material using n-hexane as mobile phase and isopropyl alcohol as modifier. Two columns of different length were used, and the influence of the volume percentage of isopropyl alcohol in the mobile phase was studied. The impact of temperature on the separation of alpha- and theta-cypermethrin was also investigated. The two enantiomers of alpha- and theta-cypermethrin were both complete resolved. The four isomers of beta-cypermethrin and the seven of eight isomers of cypermethrin were separated.